Old Remedies in the Biotechnology Age: Moore v. Regents

The California Supreme Court, by excluding a potential remedy, has eliminated many concerns generated by a lower court decision resolving rights to the cell line derived from John Moore\u27s spleen. Yet, the Supreme Court gave little attention to remedies that remain. The authors argue that a constructive trust, a remedy of considerable vintage and great flexibility, could be applied to achieve a just result in such circumstances without affecting the biotechnology world at large

Word Reading Strategy Development of Deaf and Hard-of-Hearing Preschoolers

WORD READING STRATEGY DEVELOPMENT OF DEAF AND HARD-OF-HEARING PRESCHOOLERS by Victoria Burke Siegler’s (1996) overlapping waves model of strategy development applied to reading posits that children use multiple strategies to read words from the earliest stage of reading development, that these strategies coexist over a long period of time, and that experience results in gradual change in the strategies children use and the effectiveness with which they are executed. Phonological recoding is one of the most effective early developing reading strategies and is predictive of future reading success for hearing children (Ehri, 2005; Juel & Mindencupp, 2000; Share & Gur, 1999). However, less is known regarding the extent to which young children who are deaf and hard of hearing (DHH) develop and use phonological strategies to read words. Due to technological advances such as cochlear implants and digital hearing aids, many DHH children have sufficient functional hearing to be able to perceive and represent spoken language. For these children, beginning reading strategies may resemble those of hearing children (Geers, Tobey, Moog, & Brenner, 2008; Lederberg, Schick, & Spencer, in press). The purpose of this study was to describe changes in the word reading strategies of 15 DHH preschoolers with functional hearing. These children received explicit instruction in alphabetic knowledge, phonological awareness, and early reading strategies in a year-long intervention. Instruction was videotaped and children’s overt behavior while independently reading words was coded for reading strategy and accuracy. The preschoolers used multiple reading strategies at all times including two phonological recoding strategies (segmenting phonemes only, segmenting and blending phonemes) and retrieval. Gradual change was observed in strategy choice, execution, and accuracy. Children’s use of segmenting only decreased while segmenting and blending phonemes increased between the beginning and middle of the year. Retrieval use increased between the middle and end of the year. Execution of phonological strategies gradually improved over the year. These results suggest young DHH children who have functional hearing develop and use strategies in a manner similar to hearing children and benefit from explicit instruction in the alphabetic principle

PATH (Project For Active Teaching Of History): A History Education Community Partnership

The Project for Active Teaching of History (PATH) is designed as a history education community collaborative to enhance the teaching and learning of American history. This paper will provide research findings, critical analysis, and application ideas and issues regarding PATH. The research perspectives focus on achievement of project goals and objectives in the areas of “powerful” teaching and learning and professional development and will focus on case studies regarding the project’s success in improving American history teaching and learning. PATH integrates summer seminars, colloquia, online support, project development, and various outreach projects. A second component, ePATH (electronic Project for the Active Teaching of History), has been established to provide statewide outreach through videoconferencing and additional online support

Worcester Foodscape: Analysis of Adolescent Food Choices

To learn more about the attitudes of high school students towards food, we conducted multiple focus groups and interviews with local high school students. To assess the impact of the foodscape on student food choices, we mapped food retail outlets in relation to schools and school bus stops. Student’s food choice in Worcester is complex and is influenced by advertisements, peer pressure, easy access to junk food, acculturation, and economic considerations

Mfsd14a disruption causes globozoospermia

The Mfsd14a gene, previously called Hiat1, encodes a transmembrane protein of unknown function with homology to the solute carrier protein family. To study the function of the MFSD14A protein, mutant mice (Mus musculus, strain 129S6Sv/Ev) were generated with the Mfsd14a gene disrupted with a LacZ reporter gene. Homozygous mutant mice are viable and healthy, but males are sterile due to a 100-fold reduction in the number of spermatozoa in the vas deferens. Male mice have adequate levels of testosterone and show normal copulatory behaviour. The few spermatozoa that are formed show rounded head defects similar to those found in humans with globozoospermia. Spermatogenesis proceeds normally up to the round spermatid stage, but the subsequent structural changes associated with spermiogenesis are severely disrupted with failure of acrosome formation, sperm head condensation and mitochondrial localization to the mid-piece of the sperm. Staining for β-galactosidase activity as a surrogate for Mfsd14a expression indicates expression in Sertoli cells, suggesting that MFSD14A may transport a solute from the bloodstream that is required for spermiogenesis.This work was funded by a Ford Physiology Fund Endowment (WHC).This is the author accepted manuscript. The final version is available from Bioscientifica via http://dx.doi.org/10.1530/REP-15-055

Transcribing your own qualitative data

The aim of this toolkit is to help you plan properly for transcribing, and save you time by helping you do your transcribing more efficiently. The toolkit includes advice on project planning for transcribing, tips for good quality recordings, advice on equipment and software and suggestions to help you save time while transcribing

A human infertility-associated KASH5 variant promotes mitochondrial localization

KASH5 is the most recently identified member of the KASH domain family of tail anchored, outer nuclear membrane (ONM) and endoplasmic reticulum (ER) proteins. During meiosis prophase I, KASH5 and SUN1 form a complex that spans the nuclear envelope and which links the telomeres of meiotic chromosomes to cytoplasmic dynein. This connection is essential for homologous chromosome dynamics and pairing. A recent study identified a variant in human KASH5 (L535Q) that correlated with male infertility associated with azoospermia. However, no molecular mechanism was described. Here, we report that this amino acid substitution, within the KASH5 transmembrane domain (TMD) has no predicted effects on secondary structure. However, the overall hydrophobicity of the L535Q TMD, is calculated to be lower than the wild-type KASH5, based on the GES (Goldman–Engelman–Steitz) amino acid hydrophobicity scale. This change in hydrophobicity profoundly affects the subcellular localization of KASH5. Through a series of amino acid substitution studies, we show that the L535Q substitution perturbs KASH5 localization to the ER and ONM and instead results in mistargeting to the mitochondria membrane. We suggest that this mislocalization accounts for the infertility and azoospermia phenotype in patients.Other Information Published in: Scientific Reports License: https://creativecommons.org/licenses/by/4.0See article on publisher's website: http://dx.doi.org/10.1038/s41598-021-89439-2</p

Influenza research database: an integrated bioinformatics resource for influenza research and surveillance.

BackgroundThe recent emergence of the 2009 pandemic influenza A/H1N1 virus has highlighted the value of free and open access to influenza virus genome sequence data integrated with information about other important virus characteristics.DesignThe Influenza Research Database (IRD, http://www.fludb.org) is a free, open, publicly-accessible resource funded by the U.S. National Institute of Allergy and Infectious Diseases through the Bioinformatics Resource Centers program. IRD provides a comprehensive, integrated database and analysis resource for influenza sequence, surveillance, and research data, including user-friendly interfaces for data retrieval, visualization and comparative genomics analysis, together with personal log in-protected 'workbench' spaces for saving data sets and analysis results. IRD integrates genomic, proteomic, immune epitope, and surveillance data from a variety of sources, including public databases, computational algorithms, external research groups, and the scientific literature.ResultsTo demonstrate the utility of the data and analysis tools available in IRD, two scientific use cases are presented. A comparison of hemagglutinin sequence conservation and epitope coverage information revealed highly conserved protein regions that can be recognized by the human adaptive immune system as possible targets for inducing cross-protective immunity. Phylogenetic and geospatial analysis of sequences from wild bird surveillance samples revealed a possible evolutionary connection between influenza virus from Delaware Bay shorebirds and Alberta ducks.ConclusionsThe IRD provides a wealth of integrated data and information about influenza virus to support research of the genetic determinants dictating virus pathogenicity, host range restriction and transmission, and to facilitate development of vaccines, diagnostics, and therapeutics